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Family Gnetacea
Gnetum gnemon L.
Guan zhuang mai ma teng

Scientific names Common names
Gnetum gnemon L. Bago (Tag., Bik.)
Accepted infraspecifics Bagu (Tag., Bik.)
Gnetum gnemon var. brunonianum (Griff.) Markgr. Banago (Bis.)
Gnetum gnemon var. gnemon Kunan (Davao)
Gnetum gnemon var. gracile Markgr. Nabo (Bik.)
Gnetum gnemon var. griffithii (Parl.) Markgr. Daeking tree (Eng.)
Gnetum gnemon var. tenerum Markgr. Magatungal (Lanao, Cotobato)
  Gnetum (Engl.)
  Gnetum nut (Engl.)
  Gnetum tree(Engl.)
  Joint fir (Engl.)
  Joint fir spinach (Engl.)
  Melinjo nut (Engl.)
  Paddy oats (Engl.)
  Spanish joint fir (Engl.)
  Tulip tree (Engl.)
  Two leaf (Engl.)
Gnetum genmon L. is an accepted name. KEW: Plants of the World Online

Other vernacular names
CAMOBODIA: Voe, Khalet.
CHINESE: Guan zhuang mai ma teng, Xian zhou mai ma teng.
FIJI: Bele sukau, Bui no vodre, Mosokau, Sikau, Sukau, Sukau buli, Sukau motu.
FRENCH: Gnetum a feuilles comestibles.
INDIA: Hanthu (Karbi), Ketera, Genemo (Assamese).
INDONESIA: Belinjo, Melinjo, Bagu, Bagoe, Blinjo, Eso, So, Trangkil.
JAVA: Blinjo, Ki-trangkil, Maninjo, T
MALAYSIA: Melintajau, Melinjau.
MARING: Ambiam, Ambiamtupee.
PAPUA NEW GUINEA: Tulip (Tok Pisin), Ambiam, Amgiamtupee.
SINGAPORE: Melindjo.
SOLOMON IS: Dae, Daefasia, Daemalefo.
THAILAND: Peesae, Miang, Liang, Phak, Miang phak kaniang, Liang.
VITENAMESE: Bet, Rau bep, Rau danh, Gam cay.

Gen info
- Gnetum is a genus of gymnosperms, the sole genus in the family Gnetaceae, consisting of tropical evergreen trees, shrubs and lianas. Unlike other gymnosperms, they possess vessel elements in the xylem.  (3)
- Gnetum is one of the oldest species of trees on Earth, dating back more than 200 million years. It is the sole genus with about 30 species. (6)
- The species epithet gnemon derives from "genemo", the species vernacular name in the Moluccas islands. (4)
- Gnetum gnemon derives from Latin, both from the Moluccan name of the tree, ganema.

Trees or shrubs, up to 22 m tall and 40 cm diam, without buttresses; sometimes a climbing shrub. Crown narrow, cylindrical, with a single trunk. Bark gray, marked with conspicuous or faint rings. Leaves thin, yellow when dry, tapering at both ends, but varying in shape and size, 7.5-20 × 2.5-10 cm; secondary nerves bent, joining; petiole 6-18 mm. Inflorescences solitary, axillary, simple or once branched, yellowish, 3-6 cm long, collars 3 mm broad. Flowers with broad sporophyll, twice as long as the perianth (3 mm). Sterile flowers globose, tipped or beaked, 2 mm thick, 10-15 in a ring. Inflorescences similar. Flowers 5-8 at each node, globose, tipped or beaked, 3-4 mm long, inner tube exserted by 1 mm. Fruit ripening yellow, then orange-yellow or pink, sessile (exceptionally stalked), ellipsoid, shortly apiculate, 1-3.5 cm long, almost velvety; middle envelope ribbed (Markgraf 1951). (4)

Bago is a small to medium-sized tree reaching a height of 10-15 m and a diameter up to 40 cm. Bole is cylindrical with numerous branches; crown is compact to conical in shape. Leaves are opposite, variable in size and shape, 10-20 cm long and 4-7 cm wide, oblong-ovate to lanceolate, dark green, shiny, smooth, and usually pointed at both ends. Inflorescences are borne on young shoots and older branches. Fruits are produced in small clusters, 2.3 to 3.5 cm long, oblong with smooth red skin. (The immature fruit is green turning orange-red when fully ripe.) Seed is enclosed in a fleshy covering about 1 mm thick. Seed coat is thin and brittle, separating readily from the seeds. (16)

- Native to the Philippines.
- Found in Bataan, Batangas, Camarines Provinces, Ilocos Norte, Laguna, Leyte, Mindanao,Mindoro, Palawan, Panay, Quezon, Samar, Siargao, Sibuyan.
- Also native to Assam, Bismark Archipelago, Borneo, Caroline Is., China, Fiji, Lesser Sunda Is., Malaya, Maluku, Myanmar, New Guinea, Nicobar Is., Queensland, Santa Cruz Is., Solomon Is., Sulawesi, Thailand, Tibet, Vietnam.
- Cultivated as a fruit tree.

- Yields bioactive compounds like saponins, tannins, and flavonoids.  Seeds yield an abundance of resveratrol (stilbenoid) mainly
in the form of dimers (gnetin C). (4)

- Preliminary screening of G. gnemon var. ternerum leaves showed pH of approximately 6.0, and in g/100 g, dried leaves contain 27% protein, 56.8% carbohydrates, and 36.3% total dietary fiber,and vitamin A at 3,706 µg/100 g. Mineral content was highest in N at 1.3 g/100, followed by K, P, Mg, Na, and Ca at 0.4 g, 0.3 g, 0.25 g, 0.2 g, and 0.16 g/100 g sample, respectively. Micronutrients including Zn, Mn, Cu, and Fe at 0.22, 0.21, 0.18, and 0.09 g/100 g sample were recorded in Indonesia from G. gnemon leaves. Consumption of fresh leaves will provide only 57 kcal/100 g from the edible portion, with moisture, protein, fat, carbohydrate, crude fiber, and ash contents of 81.7, 4.2, 1.5, 6.6, 4.7, and 1.3 g/100 g, respectively (Hoe & Siong, 1999). The anti-nutritional factors in leaves of G. gnemon include arsenic, phytic acid, and tannins (0.07, 1.52, and 1.52 µg/g, respectively) but no cyanide, lead, or alkaloids. Seed flour yielded protein 19.0 g/100, crude fiber 8.66/g 100, carbohydrate 64.1 g/100, and total dietary fiber 14.5 g/100 on a dry basis. (6)
- Bioassay guided study isolated 2,3-dihydroxypropyl icosanate (1), oleic acid (2) and ursolic acid (3). (see study below) (7)
- Study of EtOAC-soluble extract of leaves isolated a new phenylheptanoid, gnetumal (1), along with five known compounds (2-6). (see study below)   (11)
- Study showed seed flour to be rich in protein (19.0 g/100g), crude fiber (8.66 g/100g), carbohydrates (64.1%), total dietary fiber (14.5%), and adequate amounts of essential amino acids, fatty acids, and minerals. Antioxidant compounds such as total phenols (15.1 and 12.6 mg GAE/100g), tannins (35.6 and 16.1 mg CE/100 g), and flavonoids (709 and 81.6 mg CEQ/100 g) were higher in ethanolic extracts than aqueous extracts, respectively. FTIR spectral analysis showed seed flour to encompass major functional groups such as amines, amides, amino acids, polysaccharides, carboxylic acids, esters, and lipids. (see study below) (17)
- Seeds yield mainly dimeric stilbenoid compounds, namely: gnetin C (1), gnemonoside A (2), gnemonoside D (3), along with resveratrol (4). (see study below) (21)
- Study of fruit-bark using fractionation column chromatography results in 3 isolates A-C with presumption of containing five stilbene derivative compounds (isorhapontigenin, resveratrol, gnetin D, gnetifolin K, gnetol) and one lignan compound ((+)-lirioresinol B). (See study below) (24)
- Proximate analysis has shown that Gnetum leaves stand out for their high protein and dietary fiber content from 4.1-4.2 g/100 g fresh weight (FW) and from 4.7-6.5 g/100 g FW, respectively, while fat content is low. Leaves are rich source of copper, vitamin K and A with 0.85-12.1 ppm or 166.67-94.45% DV, 332.8 µg and 627.2 µg or 416% and 78% DV, respectively.

Studies have suggest antitumor, tyrosinase inhibitory, antioxidant, antiplasmodial, hypouricemic, hypolipidemic, angiotensin enzyme inhibitory, immunomodulatory, antidiabetic, analgesic properties.

Parts used
Roots, seeds, leaves.


- Fruit is edible. Embryo is pounded and eaten roasted; also cooked in soup. (4)
- Both fruit peel and kernel with white-yellow flesh are sweet when ripe.
- Flush and inflorescences are cook in soup or eaten as vegetable. Raw, it causes a little itching in the mouth. (4)
- Young leaves and tender tip or apex used as fresh vegetables and/or cooked. (6) Young leaves are reddish orange to light green in color with a sweet creamy taste, which turn to dark green indicating older stage or intermediate young state, and can be prepared in several dishes, cooked with eggs, in curries, coconut milk soup, or as fresh wrap with other foods. (29)
- In Java, kernels of large seeds are beaten into a flat cake, fried, eaten hot or allowed to cool and eaten like potato crisps. (4) Seed kernel processed into flour for crisps making, a home industry in Java.
- No reported folkloric medicinal use in the Philippines.
- Used for the treatment of bronchitis, arthritis, and asthma. (5)
- Roots used as antidote for poison and as medicine for malaria.
- In Malaysia, ripe fruits eaten for stomach cleansing and to prevent constipation. (36)

- Fiber: Inner bark provides a fiber, processed into making rope and cord, string bags (bilum), fishing nets and paper products. The inner bark is famous for the making of Sumba bow string.

- Wood: Soft timber is used for making boxes, tool handles, wharfing rafts and boats; branches used for making barrels.

Antiplasmodial / Cytotoxicity / Leaves:
In a preliminary study, the ethanol extract of leaves of G. gnemon showed promising antiplasmodial activity against Plasmodium falcifarum chloroquine sensitive (3D7) strain in vitro with IC50 of 29.4 µg/mL. Bioassay guided study isolated 2,3-dihydroxypropyl icosanate (1), oleic acid (2) and ursolic acid (3). Compound 3 showed highest antiplasmodial activity with IC50a of 4.0 and 6.0 µg/mL against chloroquine sensitive (3D7) and resistant (Dd2) strains of P. falcifarum, respectively. The isolated compounds showed low cytotoxicity against rat skeletal muscle (L6) and human cervical cancer (HeLa) cells. The isolated compounds may be responsible for the antimalarial activity. (7)
Antioxidant / Cytotoxicity Against HeLa Cell Line / Seeds:
Study evaluated the invitro antioxidant and cytotoxic effects of melinjo seed fractions against HeLa line.
DPPH scavenging activity of seed aqueous, ethyl acetate and N-hexane fraction was 733.12, 68.40 and 2035.70 µg/mL respectively. The ethyl acetate fraction was most active against HeLa cell line with IC50 of 45.27 µg/mL. Results showed antioxidant and cytotoxic activity against HeLa cell lines, which may be attributed to phytochemicals and stilbenoids such as resveratrol and gnetin. (8)
Decreased Serum Uric Acid / Increased HDL / Seeds / Clinical Trial: Seed extract contains trans-resveratrol (3,5,4'-trihydroxy-trans-stilbene) and other derivatives. A double-blind, randomized controlled study evaluated the beneficial effects of melinjo seed extract (MSE) in healthy adult males. The MSE significantly reduced serum uric acid and increased HDL cholesterol. MSE, trans-resveratrol, and a resveratrol dimer, gnetin C (GC) significantly inhibited AT1 receptor binding and exhibited mild agonistic activities for PPARα and PPARγ. Results showed MSE may decrease serum uric acid regardless of insulin resistance and improve lipid metabolism by increasing HCL cholesterol. (9)
Antioxidant / Leaf and Bark: Study evaluated the antioxidant properties of methanol, ethanol, boiling water, hexane and chloroform extract of leaves and bark of G. gnemon. The highest antioxidant property in the leaves was in the water extract (80.89%) while the lowest was in the hexane crude extract (61.61%) at concentration of 300 µg/ml. The highest activity in the bark was in the crude ethanol extract (72.61%), the lowest in the chloroform crude extract (46.67%) at 300 µg/ml. Results suggest a potential source of natural dietary antioxidants to replace synthesized antioxidants that might have carcinogenic effects. (10)
Antitumor / Seeds: Study evaluated the antitumor activity of melinjo seed extract (MSE) and active ingredient, gnetin C (GC), using human and murine tumor cell culture models in vitro. Antitumor activity of GC was compared with trans-resveratrol (tRV), a stilbenoid polyphenol. Results showed MSE and GC significantly inhibited the proliferation of pancreatic, prostate, breast, and colon cancer cell types (p<0.05), without affecting normal cells. GC enhanced antitumor activity more than tRV (p<0-.05). MSE and GC significantly induced apoptosis in all cancer cell line. MSE might induce apoptosis in cancer cells via caspase-3/7-dependent and -independent mechanisms. Results suggest MSC and GC have potent antitumor activity and provides evidence that MSE inhibits tumor growth, intratumoral angiogenesis, and liver metastasis in colon-26 tumor-bearing mice. (11)
effects. (10)
Gnetumal / New Phenylheptanoid / Tyrosinase Inhibitory / Leaves: Study of EtOAC-soluble extract of leaves isolated a new phenylheptanoid, gnetumal (1), along with five known compounds (2-6). Gnetumal (1) and p-coumaric acid (5) showed potent tyrosinase inhibitory activity with IC50s of 31.6 and 2.3 µM, respectively, more potent than positive control kojic acid (IC50 44.6 µM). (12)
Anti-Streptococcus mutans in Dental Caries: Streptococcus mutans is a bacteria involved in the pathogenesis of dental caries. Study investigated the effect of G. gnemon extract on bacterial viability, growth inhibition, acid production, glucosyltransferase enzyme formation, and the ability of adhesion of S. mutans ATCC 25175, in vitro. Results suggested Gnetum gnemon extract can inhibit the growth and adhesion of S. mutans in vitro. (13)
Activity Against pBSKS DNA Cleavage / Cytotoxicity / Seed Protein: Study evaluated the activity of a protein fraction isolated with DEAE and BUTYL matrices against supercoiled DNA cleavage of pBSKS. The DEAE and BUTYL protein fractions results in depletion of supercoiled DNA bands of pBSKS. Results showed the activity of protein isolates were non-toxic with IC50 >1000 g/mL. Melinjo fractionated using DEAE-650M against T47D cells with IC50 of 127.62 g/ml could be categorized as quite active and cytotoxic. (14)
Angiotensin Converting Enzyme Inhibitory Activity  / Antihypertensive / Seed: Study evaluated the angiotensin converting enzyme (ACE) inhibitory activity of melinjo seed extract and studied the molecular docking of stilbene contained in melinjo seeds. An ethyl acetate extract showed highest ACE inhibition activity (50% value of 9.77) and highest phenolic content (575.9 mg GAE/g). LC-MS analysis of EA extract yielded resveratrol, gnetin C, viniferin, and gnemonoside A/B, compounds that displayed similar physiochemical properties to lisinopril, an ACE inhibitor. In silico docking studies demonstrated they fit into the lisinopril receptors. Results showed the EA extract of seeds exhibited highest ACE inhibitory activity. Molecular docking studies suggest resveratrol dimers, gnetin C and gnemonoside A, can be considered ACE inhibitors. (15) (also see 34 below)
Seed Flour as Base for Food Products: Study evaluated Belinjau (Gnetum gnemon) seed flour was evaluated for nutritional composition, antioxidant activity, and functional properties. Inhibition of DPPH was high in ethanol extracts (48.9%) compared to aqueous extract (19.7).  The seed flour exhibited excellent nutritional, functional and antioxidant properties. It was rich in protein, dietary fiber, carbohydrates, and mineral with acceptable levels of essential amino acids and fatty acids. Results suggest the seed-flour can be modulated to develop new food formulations. (see constituents above)     (17) 
Stilbenoids / Immunomodulatory / Potentiation of T-Cell Immune Responses: Study evaluated melinjo fruit for regulatory actions on ileal immune responses. Oral administration of 50% ethanol extract at 100 mg/kg/day significantly enhanced production of Th1 cytokines IL-2 and IFN-γ irrespective of concanavalin-A stimulation. 1D and 2D NMR and MS analysis yielded glucosides gnemonoside L (5) and gnemonoside M (7) and identified five known stilbenoids, namely resveratrol (1), isorhapontigenin  (2), gnemonoside D (4), gnetins C (3) and E (6). Only the new stilbenoid, gnemonoside M (7) enhanced TH1 cytokine production in cultured PP cells. Results indicated the melinjo extract and constituent 7 potentiated T-cell dependent immune response in the ileal mucosa. (18) 
Gnetic C / Inhibitory Effect on Tyrosinase Activity and Melanin Biosynthesis: Tyrosinase is the key enzyme involved in melanogenesis. Study evaluated the in vitro inhibitory effects of gnetic C, a resveratrol dimer isolated from melinjo seeds, on tyrosinase activity and melanin biosynthesis in murine B16 cells. The inhibitory activities of gnetic C and resveratrol were almost equal against tyrosinase and melanin biosynthesis in the cells. Results showed gnetic C inhibited melanogenesis has potential as a new skin-whitening agent. The effects of gnetin C may be due to mechanisms other than direct inhibition of tyrosinase activity. (19) 
Pharmacokinetics and Safety of Resveratrol / Seed Extract: Study evaluated the pharmacokinetics of resveratrol derivatives in healthy volunteers after oral administration of 1000 mg of melinjo seed extract (MSE) powder. With repeated doses once daily for 28 days, plasma tRV (trans-resveratrol) and gnetin C concentrations with hydrolysis were in good agreement with theoretical curves. MSW powder was well tolerated up to the oral dosing of 5000 mg with no serious adverse events. (20) 
trans-Resveratrol / Prevention of Endothelial Senescence: Seeds yield mainly dimeric stilbenoid compounds, namely: gnetin C (1), gnemonoside A (2), gnemonoside D (3), along with trans-resveratrol (4). trans-Resveratrol has been reported to have antiaging anticancer, and antidiabetic effects, as well as a calorie restriction mimetic. Study evaluated the effects of the four main stilbenoid derivatives of a G. gnemon seed endosperm ethanolic extract on endothelial senescence. Administration of the ethanolic extract in STZ-induced diabetic mice increased SIRT1 and decreased endothelial senescence. In senescent human umbilical vein endothelial cells, endothelial senescence was inhibited by 4, which increased the expression of endothelial nitric oxide synthase and SIRT1. Results indicated the extract of seeds inhibited endothelial senescence, suggesting trans-resveratrol plays a critical role in prevention of endothelial senescence. (21) 
Hyperuricemic / Leaves and Peel: Study evaluated the effect of melinjo leaves and peels extract to reduce uric acid on induced-hyperuricemia male rats model.  Induction was done by giving high purine diet, melinjo seed crude drug (emping) 4.5 g/kbw. The ethanol leaves extract of melinjo 36 mg/kbw decreased uric acid levels up to 61.04%, while the ethanol peel extract at 13 mg/kbw decreased uric acid levels up to 31.25%. Results showed melinjo seed crude extract can increase blood uric acid level in rats up to 4.65 ng/dL. Ethanol leaves and peels extracts of melinjo could decrease uric acid blood levels in hyperuricemic rats. (22) 
Antidiabetic / Antioxidant / Seed Protein Hydrolysate: Study evaluated proteins hydrolyzed from melinjo seeds at different stages of maturity--green (GM), yellow (YM), red (RM)--for antioxidant and antidiabetic effects. Degree of hydrolysis ranged from 52-84%. The green hydrolysate (GMH) exhibited significantly higher (p<0.05) free radical scavenging activities. In invitro antidiabetic testing, GMH was found more effective in a-amylase and α-glucosidase inhibitory activities. Results showed antioxidant and antidiabetic activity of hydrolyzed GM protein has potential for use as natural nutraceuticals. (23) 
Antioxidative / Cytotoxicity Against Leukemia Cells / Fruit Rind: Study of fruit-bark using fractionation column chromatography results in 3 isolates A-C along with five stilbene derivative compounds and one lignan compound. LC-ESI-MS showed gnetol and (+)-lirioresinol B was successfully prenylated. The prenylated stilbene showed comparable antioxidant effect and moderate activity against P-388 murine leukemia B cells. The inhibition of the bioactivity test provides a reasonable mechanism for the potent cancer chemopreventive activity of prenylated stilbene compounds with potential as a valuable agent for treatment of diseases. (see constituents above) (24)
Analgesic / Synergism with  Artocarpus altilis / Leaves: Sukun (Artocarpus altilis) and Melinjo (Gnetum gnemon) leaves are expected to have analgesic properties because they contain bioactive compounds that can inhibit biosynthesis of prostaglandins. Study evaluated the analgesic effect of the combination of the two plants in male mice using writhing test. Results showed the extracts at dose ratio of 150:50 (sukun:melinjo respectively) showed to be the most effective combination, with comparable analgesic effects to mefanamic acid. (25)
HMG-CoA Reductase Inhibitory Activity / Anti-cholesterol / Seed Extract: Study evaluated by in vitro and in silico methods the antihypercholesterolemic potential of Melinjo seed extracts through HMG-CoA reductase inhibitory activity. A dichloromethane extract demonstrated highest inhibitory activity against HMG-CoA reductase with IC50 of 0.40 µg/mL. UPLC-MS analysis showed the extract contained trans-resveratrol, piceid, gnetin C, gnetol, isorhapontigenin, ɛ-viniferin, gnemonol L, and gnemonol M. Results showed the seed extract possess strong HMG-CoA reductase inhibitory activity. The resveratrol dimer has potential as a lead compound to design/synthesize anti-cholesterol agents. (26)
UVB-Protective Activity / Antioxidant / Stem Bark: Study of stem bark isolated three phenolic compounds, namely 3,4-dimethoxychlorogenic acid (1), resveratrol (2) and 3-methoxyresveratrol (3). Each compound showed radical scavenging activity and significant UV-B protection. Resveratrol and methoxyresveratrol showed maximum UV-B protections (SPF 8.03 and 12.34, respectively)  at 50 µg/mL. (27)
Effect of Food Processing on Resveratrol and Phenolic Contents of Seeds: Study evaluated the effects of food processing on trans-resveratrol and total phenolic compound levels in melinjo seeds. Results showed trans-resveratrol (tRV) and total phenolic content (TPC) in Melinjo powder decreased from 35% to 10% (tRV)and 15% to 4% (TPC) after 20 min of roasting. In fried empings, it decreased 60%, 68%, and 92% (tRV) and 41%. 45%, and 97% (TPC) after 5, 15, and 30 min of boiling. Results showed significant changes in tRV and TPC in Melinjo seeds before and after various cooking processes (roasting, boiling, and frying). (28)
Antidiabetic / Chlorophyllin /Leaves: G. gnemonm var. tenerum leaves contain 3 components, protein, fiber, and chlorophyll with a high potential for antidiabetic properties. A study has shown that alanine, arginine, and glutamine stimulated insulin secretion while chlorophyllin derived from chlorophyll exhibited an antidiabetic effect similar to metformin. Fiber also attenuates absorption of sugar in the bloodstream. Liang leaves, with its high protein and fiber, as well as chlorophyll and its water-soluble derivative chlorophyllin, have been investigated for anti-diabetic properties, and results have suggested potential for a significant positive effect for diabetic patients as as future antidiabetic product. (29)
Gnetol / Potent Tyrosinase Inhibitor / Suppression of Melanin Biosynthesis: Gnetol (2,3',5',6-tetrahydroxy-trans-stilbene), a naturally occurring compound particularly found in genus Gnetum, had a strong inhibitory effect on murine tyrosinase activity. Gnetol (IC50 4.5 µM) was stronger than kojic acid (IC50 139 µM) as standard inhibitor for murine tyrosinase activity.  Gnetol also significantly suppressed melanin biosynthesis in murine B16 melanoma cells. (30)
Increased Skin Absorption / Ionic Liquid-Gnetum gnemon Seed Extract Loaded Lipid Nanoparticles: Study evaluated the skin absorption of ionic liquid-melinjo seed extract (IL-MSE) loaded solid lipid nanoparticles (SLN) formulated using glyceryl monostearate as lipid ingredient among others, including 10% melinjo seed extract. The IL-MSE loaded to SLNs could improved the absorption of trans-resveratrol through the skin. (31)
Anti-Acne / Antibacterial Against Propionibacterium Acnes / Leaves: Propionibacterium acnes play a role in the occurrence of inflammation in acne. Study evaluated the effectiveness of melinjo leaf extract on the growth of bacteria Propionibacterium acnes using melinjo leaf extract using 96% ethanol solvent. Results showed concentrations of 2.5%, 5%, 7.5%, and 10% could inhibit growth of bacteria Propionibacterium acnes with inhibition zones from 6.13, 6.68, 7.92, and 10.94 mm. respectively. The higher the concentration of leaf extract, the higher the resistance of bacteria. (32)
Skin Whitening Efficacy / Seed Extract Loaded Lipid Particle Gel: Melinjo seed extract has potential as skin-whitening agent because it contains trans-resveratrol and its derivatives, which inhibit tyrosinase in melanogenesis process. Study evaluated the skin safety and whitening efficacy of MSE-loaded lipid particle gel in healthy human subjects. The test product did not induced skin irritation effect. The skin melanin index was statistically significantly decreased (p<0.05) after 28 days. Results suggest application of MSE-loaded lipid particle gel can brighten the skin, without skin irritation under normal conditions of use. (33)
Potential ACE Inhibitory / Gnemonol K / Seeds: Study evaluated the potential ACE (angiotensin coverting enzyme) inhibitory activity of several compounds isolated from Gnetum gnemon seeds by using in silico method. In the study, several compounds isolated from the seeds were evaluated for ACE inhibitory activity through molecular docking study and molecular dynamics simulations. Results showed that resveratrol, gnetol, isorhapontigenin, gnetin C, trans-ε-viniferin, gnemonol K, gnemonol M and aglycone of gnemonoside B exhibited values lower than or close to lisinopril, captopril, and enalapril. Gnetin C showed the highest affinity for ACE among other ligands. Results suggest compounds from melinjo seed showed potential ACE inhibitory activities, with gnemonol K showing most potential for ACE inhibitory activity. (34)
Antimicrobial Organic Soap / Peel: Study reported on the production of organic soap bars from melinjo peel extract, the quality testing, and antimicrobial analysis against selected microbes. Results showed the quality test fit with INS 2016 for all criteria except for unsaponified fatty acid in the cold process soap bar. Inhibition zone of organic soap sequentially decreased against the growth of Trichophyton mentagrophytes, Staphylococcus aureus, and Staphylococcus epidermis. Results suggest the organic soap with melinjo peel extract shows good potential as an antimicrobial soap. (35)
Antidiabetic / Effect on Glucose and Insulin Levels / Seed: Study evaluated Melinjo seed extract activity in a diabetes mellitus rat model. Melinjo seed extract was administered at doses of 200, 400, 600 mg/kbw orally for 21 days in diabetic rats. Results showed MSE at 600 mg/kbw could reduce serum glucose levels from 372.22 to 273.70 mg/dL and increase insulin levels from 68.81 to 253.11 µM/mL, increase the density of Langerhans islet from 73.49 to 114.80. Results suggest melinjo seed extract has antidiabetic activities as evidenced by improvement in glucose and insulin levels, increase in Langerhans density, and Langerhans PDX-1 expression. (38)
Potential for Extracting Virgin Coconut Oil / Protease from Fruit Peel Waste: Study evaluated the potential of agricultural waste, paddy oats fruit peel, as a source of protease especially for extracting virgin coconut oil from coconut milk. Protease is an important biocatalyst, an inexpensive choice for hydrolysing peptide bonds for industrial use. Paddy oats protease showed high performance for extracting VCO with high yield of 25.7% and good quality oil with free fatty acids of 0.092%, density of 0.924 g/mL, and a consistently high rank for all evaluated sensory parameters. Results suggest paddy oats fruit peel, especially the ripe peel, has great potential as source of protease for extracting VCO.       (39)
Protease for Improving Meat Tenderness / Fruit Peel: Study evaluated the potential of an agricultural waste, paddy oats (Gnetum gnemon ) fruit peel as source of protease, particularly for use as meat tenderizer. Effect of paddy oats was compared to commercial papain, commercial bromelain, and control. Panelists scored the meat sample treated with paddy oats protease extract as much more tender (7.4) than control (5.8) and no different from commercial papain and bromelin at 7.3 and 7.7, respectively. The paddy oats protease also ranked high for all evaluated parameters. Results showed high potential as a meat tenderizer. (40)
Gnetin C and trans-Resveratrol / Inhibitors of Breast Cancer Cells MCF-7: Molecular docking evaluated the binding affinity of Gnetic C and trans-resveratrol as active compounds of melinjo seeds to inhibit breast cancer cells Michigan Cancer Foundation (MCF-7). Results showed Gnetin C and trans-resveratrol could bind the same amino acid as natural ligan of MCF-7 and can bind the protein acid of MCF-7. Study suggests Gnetic C and trans-resveratrol are potential anticancer and chemopreventive because they can inhibit some amino acids of cancer cell MCF-7. (41)
Copper Nanoparticles / Leaves: Study reports on the synthesis of copper NPs using melinjo leaves extract as bioreductor. Copper nanoparticle synthesis conditions can be used routinely in the laboratory for further investigation as topical antibacterial candidate. (42)
Antibacterial Against Staphylococcus aureus / Leaves: Study evaluated the MIC and MBC of melinjo leaves extract on the growth of Staphylococcus aureus bacteria. Result showed the melinjo leaf cancer has antibacterial effect against S. aureus with MIC at concentration of 640 mg/ml. MBC was not obtained because the extract clotted above 1280 mg/ml concentration. (43)


October 2022

IMAGE SOURCE: Photograph: Melinjo (Gnetum gnemon) / gbohne / Berlin, Germany / CC by SA 2.0 / click on image to go to source page / WIKIMEDIA COMMONS
OTHER IMAGE SOURCE: Photograph / Gnetum gnemon seeds / Photograph taken with financial support from the Rufford Small Grants Foundation / Copyright © 2013 by P.B. Pelser & J.F. Barcelona (contact: [ref. DOL62586] / Non-Commercial Use / click on image to go to source page /
OTHER IMAGE SOURCE: Photograph: Gnetum gnemon L. fruits and seeds / click on image to go to source page / © Seeds Gallery
OTHER IMAGE SOURCE: Photograph / Gnetum gnemon leaf / Copyright © 2018 by P.B. Pelser & J.F. Barcelona (contact: [ref. DOL137361] / Non-Commercial Use / click on image to go to source page /
OTHER IMAGE SOURCE: Photograph: Gnetum gnemon (Jacq.) buko / Tracy Slotta Hurst @ USDA-NRCS PLANTS Database / USDA

Additional Sources and Suggested Readings
Gnetum gnemon: Gnetaceae / Harley I Manner, Craig R Elevitch / Species Profiles for Pacific Island
Gnetum gnemon / KEW: Plants of the World Online
Gnetum / Wikipedia
Gnetum gnemon / The Gymnosperm Database
Gnetum gnemon Linn: A Comprehensive Review on its Biolotgical, Pharmacological, and Pharmacognostical Potentials / Barua CC, Haloi P, Barua I C / International Journal of Pharmacognosy and Phytochemical Research, 2015; 78(3): pp 531-539 / ISSN: 0975-4873
A comprehensive review on nutritional contents and functional properties of Gnetum genmon Linn.
/ Nata Anisong, Panupong Puttarak et al / Food Sci Technolo, 2022; 42 / DOI: 10.1590/fst.100121
Antiplasmodial Activity of Gnetum gnemon Leaves and Compounds Isolated from them / Partha P Dutta, Manobjyoti Bordoloi, Sonali Roy, Bhaskkar Mazumder et al / NPC: Natural Products Communications, 2018; 13(10): pp 1263-1265 / DOI: 10.1177/1934578X1801301007
Antioxidant and Cytotoxic Acitivities of Melinjo (Gnetum gnemon L.) Seed Fractions on HeLa Cell Line in Vitro / Asep Sukohar, Suharyani, Sutyarso, Hendri Busman, Nuning Nurcahyani, and Evi Kurniawaty / Pharmacognosy Journal, 2022, 14(3): pp 559-564 / DOI: 10.5530/pj.2022.14.71
Melinjo (Gnetum gnemon L.) Seed Extract Decreases Serum Uric Acid Levels in Non-Obese Japanese Males: A Randomized Controlled Study / Hiroyuki Konno, Yoshiaki Kanai, Tajuki Shirasawa et al / Evidence-Based Complementary and Alternative Medicine, Vol 2013; Artilce ID 589169 / DOI: 10.1155/2013/589169
Antioxidant properties of leaf and bark crude extracts of Gnetum gnemon L. (Gnetaceae) 2009
/ Syahida A, M Radzali, W Dayana, R Johari et al / AGRIS / Universiti Putra Malaysia
Antitumor activity of melinjo (Gnetum gnemon L.) seed extract in human and murine tumor models in vitro and in a colon-26 tumor-bearing mouse model in vivo / Narayanan K Narayanan, Kazuhiro Kunimasa, Yukio Yamori, Bhagavathi Narayanan et al / Cancer Medicine, 4(11): pp 1767-1780 /
DOI: 10.1002/cam4.520
A new phenylheptanoid from the leaves of Gnetum gnemon L. / Tho Huu Le, Truong  Nhat Van Do, Mai Thanh Thi Nguyen et al / Natural Products Research, 2021; 35(21): pp 3999-4004 /
DOI: 10.1080/14786419.2020.1753055
The Effects of Gnetum gnemon Extract on Streptococcus mutans: In vitro study / Nattapon Rotpenpian, Suwanna Jitpukdeebodintra / Khon Kaen University Dental Program, Aug 2021; 24(2) / eISSN: 2730-1699

Melinjo (Gnetum gnemon) Seed Protein Activity Against pBSKS DNA Cleavage and its Cytotoxicity in T47D and 4T1 Cells / KnE Medicine: The International Conference of Medicine and Health: pp 517-533
Angiotension converting enzyme inhibitory activity of melinjo (Gnetum gnemon L.) seed extracts and molecular docking of its stilbene constituents / Abdul Mun'im, Muhammad Ashar Munadhil, Nuraini Puspitasari, Azminah, Arry Yanuar / Asian Journal of Pharmaceutical and Clinical Research. 2017; 10(3) / DOI: 10.22159/ajpcr.2017.v1013.16108
Bago: Gnetum gnemon Linn. / Rafael T Cadiz, Helen B Florido / Research Information Series on Ecosystems, 2001; 13(2)
Evaluating belinjau (Gnetum gnemon L.) seed flour quality as a base for development of novel food products and food formulations / Rajeev Bhat, Nabilah Binti Yahya / Food Chemistry, 2014; Vol 156: pp 42-49 / DOI: 10.1016/j.foodchem.2014.01.063
Stilbenoids from the Melinjo (Gnetum gnemon L.) Fruit Modulate Cytokine Production in Murine Peyer's Patch Cells Ex Vivo / Hikaru Kato, Masayuki Samizo, Tomihisa Ohta et al / Planta Med, 2011; 77(10): pp 1027-1034 / DOI: 10.1055/s-0030-1250742
Inhibitory Effect of Gnetin C, a Resveratrol Dimer from Melinjo (Gnetum gnemon), on Tyrosinase Activity and Melanin Biosynthesis / Miyako Yanagihara, Maiko Yoshimatsu, Ken Hashimot et al / Biological and Pharmaceutical Bulletin, 2012; 35(6): pp 993-996 / DOI: 10.1248/bpb.35.993
Pharmacokinetics and Safety of Resveratrol Derivatives in Humans after Oral Administration of Melinjo (Gnetum gnemon L.) Seed Extract Powder / Hiroko Tani, Susumu Hikami, Kazutaka Higaki et al / J Agri Food Chem., 2014; 62(8): pp 1999-2007 / DOI: 10.1021/jf4048435
trans-Resveratrol in Gnetum gnemon Protects against Oxidative-Stress-Induced Endothelial Senescence
/ Hidetaka Ota, Masahiro Akishita, Ysuyoshi Ouchi et al / J Nat Prod., 2013; 76(7): pp 1242-1247 /
DOI: 10.1021/np300841v
The Effect of Melinjo (Gnetum gnemon L.) Leaves and Melinjo Peel Extracts on Induced Hyperuricemia Male Rats Model / Nia K Sari, Andreanus A Soemardji, Irda Fidrianny / Journal of Medicine & Health, 2019; 2(4) / DOI: 10.28932/jmh.v2i4.1840
Revealing Antioxidant and Antidiabetic Potency of Melinjo (Gnetum gnemon) Seed Protein Hydrolysate at Different Stages of Seed Maturation / Anang Supriyadi, Laras Sekar Arum, Tri Agus Siswoyo  et al / Current Research in Nutrition and Food Science / DOI: 10.12944/CRNFSJ.7.2.17
Antioxidative and cytotoxic effects of prenylated stilbene derivative-rich Melinjo (Gnetum gnemon L.) fruit rind / Antonius Herry Cahyana, Bayu Ardiansah / AIP Conference Proceedings, 2016; 1729: 020057 /
DOI: 10.1063/1.4946960
Analgesic activity of the combined extract of Sukun (Artocarpus altilis) and Melinjo (Gnetum gnemon L.) leaves in mice / Mashuri Yusuf, Lilik Koernia Wahidah, Mohammad Kanedi et al / GSC Biological and Pharmaceutical Sciences, 2020; 13(1) / eISSN: 2581-3250 / DOI: 10.30574/gscbps.202013.1.344
HMG-CoA Reductase Inhibitory Activity of Gnetum gnemon Seed Extract and Identification of Potential Inhibitors for Lowering Cholesterol Level / Kholid Abdul Hafidz, Nuraini Puspitarsi, Abdul Mun'im et al / Journal of Young Pharmacists, 2017; 9(4): pp 559-565 / DOI: 10.5530/jyp.2017.9.107
Some phenolic compounds from stem bark of melinjo (Gnetum gnemon) and their activity test as antioxidant and UVB-protection / Sri Atun, Retno Arianingrum, Niwa Masatake / PROCEEDING: JSChem-ITB-UKM-2007
Effects of Food Processing on Resveratrol and Total Phenolic Content in Melinjo (Gnetum gnemon L.) Seeds / Islamudin Ahmad, Desta Andriyani, Abdul Mun'im et al / Pharmacognosy Journal, 2018; 10(6): pp 1096-1100 / DOI: 10.5530/pj.2018.6.186
The potential antidiabetic properties of Lian (Gnetum gnemon var. tenerum) leaves / Anunya Suksanga, Sunisa Siripongvutikorn, Chutha Takahashi Yupanqui, Rattana Leelawattana / Food Sci. Technol., 2022, 42 / DOI: 10.1590/fst.64522
Gnetol as a Potent Tyrosinase Inhibitor from Genus Gnetum / Kenji Ohbuchi, Toshiyuki Tanaka, Yoshinori Nozawa et al / Biochemistry & Molecular Biology Preliminary Communication, 2003; 67(3): pp 663-665 /
DOI: 10.1271/bbb.67.663
Inn vitro penetration activity of ionic liquid-Gnetum gnemon seed extract loaded solid lipid nanoparticles / Elsa Trinovita, Maesya Rachmawati, Sutriyo Sutriyo, Abdul Munim / Research Article, 2019; 9(10) /
DOI: 10.7324/JAPS.2019.91002
Test Effectiveness Antimicrobial Extract Ethanol Leaves Melinjo (Gnetum gnemon L.) on Growth of Bacteria Propionibacterium acnes / Resva Meinisasti, Wenti Puspita, Raden Sunita / Proceedings of the Ist International Conference on Inter-Professional Health Collaboration (ICIHC 2018) /
DOI: 10.2991/icihc-18.2019.25
The Study of Safety anbd Skin Whitening Efficacy of Melinjo (Gnetum gnemon L.) Seed Extract-Loaded Lipid Particle Gel / Pharmacognosy Reserch, 2018; 10(4): pp 432-436 / DOI: 10.4103/pr.pr_17_18
Analysis of Compounds Isolated from Gnetum gnemon L. Seeds as Potential ACE inhibitors through Molecular Docking and Molecular Dynamics Stimulation / Muhammad Aranza Triputra, Arry Yanuar / Journal of Young Pharmacists, 2018; 10(2S): pp 32-39 / DOI: 10.5530/jyp.2018.2s.7
Preparation and Antimicrobial Activity Analysis of Organic Soap Bar Containing Gnetum gnemon Peel Extract / Sri Handayani, Indyah Sulistyo Arty, Melati Khairuddean et al /
MOLEKUL, 2021; 16(3) /
pISSN: 1907-9761 / eISSN: 2503-0310
The Use of Medicinal Plant Species by the Temuan Tribe of Ayer Hitam Forest, Selangor, Peninsular Malaysia / I Faridah Hanum, Nurulhuda Hamzah / Pertanika J Trop Agric Sci., 1999; 22(2): pp 85-94 /
ISSN: 1511-3701

SR: Effect of Resveratrol in Melinjo Seed (Gnetum gnemon L.) Extract on Type 2 Diabetes Mellitus Patients and Its Possible Mechanism: A Review /  Ariyanto FF. Danil AS, Rohmawaty E, Sujatmiko B, Berbudi A /
Current Diabetes Reviews, 2022 / DOI: 10.2174/157339981866622022281 / PMID: 35227186
Potential of Gnetum gnemon L. Seed Extract Against Insulin Levels and PDX Expression in Diabetes Mellitus Rat Model / Rosni Rosni, I Nyoman Ehrich Lister, Edy Fachrial et al / IEEE 2021: International Conference on Health, Instrumentation & Management, and Natural Sciences, 2021: pp 1-5 /
DOI: 10.1109/InHeNce52833.2021.9537215
Protease from Paddy Oats (Gnetum gnemon L.) Seed Peel and Its Potential for Extracting Virgin Coconut Oil / C Indarto, Pomin Li / International Research Journal of Advanced Engineering and Science, /
eISSN: 2455-9024
Improving meat tenderness by using protease extract from paddy oats (Gnetum gnemon) fruit peel / Cahyo Indarto, Shyang-Chwen Sheu, Pomin Li / Proceedings of the 9th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering (ISMAB), May 2019, Jeju, Korea
Molecular Docking of Gnetic C and Trans-Resveratrol of Melinjo Seeds (Gnetum gnemon L.) Used as Inhibitors of Breast Cancer Cells MCF- 7 / Kintan Nur Romadhona, Nenden Aulia Shifa, Asmiyenti Djaliasrin Djalil / International Journal of Health and Medical Sciences, 4(3): pp 58-63 /
DOI: 10.20469/ijhms.40001-3
Copper nanoparticles synthesis optimization using melinjo (Gnetum gnemon L.) leaves extract and beta cyclodextrin as a stabilizer / H A Wisnuwardhani, R D Shafira et al / Medical Technology and Environmental Health, 2020 / eBook ISBN: 9781003016700
Antibacterial effect of Gnetum gnemon L. leaves extract on Staphylococcus aureus
/ Paul J Dayoh, Endang Isbandiati, Titien Rahayu / Journal of Widya Medika Junior,, 2021; 3(2)

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