Family Rutaceae
Kabuyaw
Citrus hystrix

KAFIR LIME
Common names   
Amongpong (C. Bis.)  Kamuntai (Bik.) 
Amontau (C. Bis)  Kapitan (Ilk., Ibn.) 
Buyak (Tag.)  Kobot (Tag.) 
Buyog (Tag.)  Kolison (Bis.) 
Daruga (Sul.)  Kolobot (Tag., Bis.) 
Duroga (Sul.)  Kolong-kolong (Tag.)
Kabog (Bik.)  Kopalian (C. Bis.) 
Kabuan (Tag.)  Malatbas (Sbl.) 
Kabugau (Tag.)  Mayagarin (C. Bis.) 
Kolo-oi (C. Bis.) Muntai (Sub.) 
Kamugau (Ilk.) Pinukpuk (Klg.) 
Kamukau  (Ilk.) Piris (Pang.) 
Kamulan (Ilk.)  Kafir lime (Engl.)


Image links

Botany
Small, slender, erect tree. Leaves are smooth, oblong, 10-15 cm long, 3-5 cm wide. Flowers are cream-colored, in fascicles upon short, green axillary spikes. Fruit is flat, globose, up to 10 cm diameter; many seeded. Flesh is white and astringently sour. Rind is thick, lemon yellow.

Distribution
Native; common in primary and secondary forests, at low and medium altitudes.

Constituents
Rind: volatile oil, 4%; citral, 40%.
Leaves: volatile oil, 0.08%.
From the essential oil: Limonene (most abundant), B-pinene, terpinen-4-ol and a-terpinol.
Three coumarins have been reported: bergamottin, oxypeucedanin and 5-[(6',7'-dihydroxy-3',7'-dimethyl-2-octenyl)oxy] psoralen.


Parts used and preparation
Leaves and fruits.

Uses
Folkloric
Aromatic baths: Squeeze fruit and juice to gogo (for shampoo) or water (bath).
Nausea and fainting: Inhale oil from rind.
Peel is a tonic ingredient; rind used in treatment headache and woms in children.
In Malaysia, used for stomachaches and dyspepsia.
Fruit juice is expectorant and antidandruff.
With ginger and other aromatics, used to treat postpartum septicemia.
Others
Flavor - Squeeze rind to extract oil.
Volatile oil used in perfumery, toiletries, handwash and shampoo.
Leaves used to season food.
Leaves used in preparation of Thai and Malay dishes.
Juice of fruit used as insecticide. (Read)


Studies
Insect Repellent:
Essential oil effective in repelling mosquitoes, but less than tumeric or citronbella grass.
Coumarins:
Study isolated three known coumarins from CH as inhibitors of both lipopolysaccharide and interferon-y-induced nitric oxide generation in in Mouse Macrophage RAW 264.7 Cells.
• Anti-Tumor:
Glyceroglycolipids from Citrus hystrix, a Traditional Herb in Thailand, Potently Inhibit the Tumor-Promoting Activity of 12-O-Tetradecanoylphorbol 13-Acetate in Mouse Skin: Study isolated two glyceroglycolipids from the leaves of Citrus hystrix. Both showed to be potent inhibitors of tumor promoter-induced Epstein-Barr virus (EBV) activation. The anti-tumor promotion may involved the inhibition of arachidonic acid cascade.
• Anti-Microbial: (1) Study of fresh and oil extracts fifteen medicinal herbs were tested for potential use as natural antimicrobial additives in foods. Tested against B cereus, S typhi and S aureus, six , including Citrus hystrix, showed high activities on first screening. C. hystrix, together with A. ascalonicum and C aurantifolium were selected as potential antimicrobial food additives. (2) Study showed the ethyl acetate extract of kaffir lime (C hystrix) peel showed broad spectrum inhibition against all Gram-positive bacteria, yeast and molds including S aureus, B cereus, Listeria monocytogenes, Saccharomyces cerevisae and A fumigatus.
• Flavonoid / Antioxidant: Study compared frresh use with effects of boiling and deep-fat frying on the leaf of Citrux hystrix's phenolic . flavonoid contents and antioxidant capacities. Results showed boiling decreased total antioxidant capacity and that the method of procerssing can significantly affect the flavonoid content and their total antioxidant capacities.
• Antiproliferative / Anticancer: Study on the anti-proliferative activity of essential oil
• Antioxidant / Free Radical Scavenging Activity: Study on 10 methanolic extracts from various medicinal plants commonly used in Thai traditional medicine, the extract from leaves and peels of Kaffir lime exerted the strongest effect on production of the hydroxyl radical (OH).

Caution
• Photodermatitis: Occasional reports of photodermatitis from rubbing the juice onto the skin for insect bites (eMediine)

Availability
Wild-crafted.
Kaffir Lime Oil: CyberMarket



Additional Sources and Suggested Readings
(1)
Citrus hystrix DC. / ICS.UNIDO / Medicinal and Aromatic Plants
(2)
Identification of Coumarins from the Fruit of Citrus hystrix DC as Inhibitors of Nitric Oxide Generation in Mouse Macrophage RAW 264.7 Cells / Akira Murakami, Guanxin Gao et al / J. Agric. Food Chem., 1999, 47 (1), pp 333–339 / DOI: 10.1021/jf980523e
(3)
Glyceroglycolipids from Citrus hystrix, a Traditional Herb in Thailand, Potently Inhibit the Tumor-Promoting Activity of 12-O-Tetradecanoylphorbol 13-Acetate in Mouse Skin / Akira Murakami, Yoshimasa Nakamura, Koichi Koshimizu, Hajime Ohigashi
J. Agric. Food Chem., 1995, 43 (10), pp 2779–2783 / DOI: 10.1021/jf00058a043
(4)
PRELIMINARY STUDY OF ANTIMICROBIAL ACTIVITIES ON MEDICINAL HERBS OF THAI FOOD INGREDIENTS / Chaisawadi, S., Thongbute, D., Methawiriyasilp, W., Pitakworarat, N., Chaisawadi, A., Jaturonrasamee, K., Khemkhaw, J. and Tanuthumchareon, W. 2005. Acta Hort. (ISHS) 675:111-114
(5)
Effect of processing on the flavonoid content and antioxidant capacity of Citrus hystrix leaf / Chaniphun Butryee; Pongtorn Sungpuag; Chureeporn Chitchumroonchokchai / DOI: 10.1080/09637480903018816 /
(6)
Antimicrobial activities of essential oils and crude extracts from tropical Citrus spp. against food-related microorganisms / Sumonrat Chanthaphon et al / Songklanakarin Journal of Science and Technology
(7)
Antioxidative and Free Radical Scavenging Activities of Some Plants Used in Thai Folk Medicine / N. Hutadilok-Towatana, P. Chaiyamutti, K. Panthong, W. Mahabusarakam and V. Rukachaisirikul / Summary Pharmaceutical Biology, 2006, Vol. 44, No. 3, Pages 221-228 / DOI 10.1080/1388020060068559


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